dacC [2017-8-11 16:2:30]

penicillin-binding protein 4A, D-alanyl-D-alanine carboxypeptidase

locus: BSU18350

pI: 5.41

mw: 52.72 kDa

protein length: 491 aa

gene length: 1473 bp

function: carboxypeptidase

product: penicillin-binding protein 4A, D-alanyl-D-alanine carboxypeptidase

essential: no

synonyms: pbp

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 1,998,340 → 1,999,815

The protein

Catalyzed reaction/ biological activity

• cleavage ofD-Ala-D-Ala interpeptide bridges in peptidoglycan [Pubmed|19758330]

Structure

• [PDB|1W5D], [PDB|2J9P] (complex with a peptidoglycan mimetic peptide)

Expression and Regulation

Operons

• genes: [gene|993A863ED80BA5970C9145A35D407834B322C9DC|galM]-[gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]
  description: [Pubmed|9733705]

  sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|9733705], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|10482513], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

Biological materials

Mutant

• MGNA-B075 (dacC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1074 NBRP B. subtilis, Japan]

References

Reviews

• 18266855

Original publications

• 9733705,10482513,19758330,14731276,17582436,9864321,21821766,22029692,23560856,26460848